| First Author | Oliveira VG | Year | 2011 |
| Journal | Eur J Immunol | Volume | 41 |
| Issue | 5 | Pages | 1249-55 |
| PubMed ID | 21469093 | Mgi Jnum | J:175409 |
| Mgi Id | MGI:5285493 | Doi | 10.1002/eji.201040896 |
| Citation | Oliveira VG, et al. (2011) Sub-optimal CD4+ T-cell activation triggers autonomous TGF-beta-dependent conversion to Foxp3+ regulatory T cells. Eur J Immunol 41(5):1249-55 |
| abstractText | Classical in vitro Treg conversion assays, which rely on optimal T-cell activation in the presence of exogenous TGF-beta, induce Foxp3 expression at a frequency far above that which is observed in vivo in Treg-dependent models of oral or transplantation tolerance. We have found that suboptimal murine T-cell activation in vitro results in induction of Foxp3 expression, in the absence of exogenous TGF-beta, at a frequency similar to that which we found in vivo upon anti-CD4-induced transplantation tolerance. We show that TCR triggering with either low-dose anti-CD3 or low-dose agonist peptide, as well as down-modulation of the TCR signal with non-depleting anti-CD4, promotes TGF-beta production by T cells, an event that precedes Foxp3 expression and is Foxp3 independent. These findings support the view that sub-immunogenic regimens lead to dominant tolerance as a result of T-cell intrinsic properties. |
