| First Author | Vidal RL | Year | 2007 |
| Journal | Mol Cell Neurosci | Volume | 35 |
| Issue | 3 | Pages | 501-12 |
| PubMed ID | 17532644 | Mgi Jnum | J:123202 |
| Mgi Id | MGI:3717501 | Doi | 10.1016/j.mcn.2007.04.008 |
| Citation | Vidal RL, et al. (2007) Marlin-1 and conventional kinesin link GABA(B) receptors to the cytoskeleton and regulate receptor transport. Mol Cell Neurosci 35(3):501-12 |
| abstractText | The cytoskeleton and cytoskeletal motors play a fundamental role in neurotransmitter receptor trafficking, but proteins that link GABA(B) receptors (GABA(B)Rs) to the cytoskeleton have not been described. We recently identified Marlin-1, a protein that interacts with GABA(B)R1. Here, we explore the association of GABA(B)Rs and Marlin-1 to the cytoskeleton using a combination of biochemistry, microscopy and live cell imaging. Our results indicate that Marlin-1 is associated to microtubules and the molecular motor kinesin-I. We demonstrate that a fraction of Marlin-1 is mobile in dendrites of cultured hippocampal neurons and that mobility is microtubule-dependent. We also show that GABA(B)Rs interact robustly with kinesin-I and that intracellular membranes containing GABA(B)Rs are sensitive to treatments that disrupt a protein complex containing Marlin-1, kinesin-I and tubulin. Finally, we report that a kinesin-I mutant severely impairs receptor transport. We conclude that Marlin-1 and kinesin-1 link GABA(B)Rs to the tubulin cytoskeleton in neurons. |
