| First Author | Brehm MA | Year | 2005 |
| Journal | J Immunol | Volume | 175 |
| Issue | 8 | Pages | 5043-9 |
| PubMed ID | 16210607 | Mgi Jnum | J:119124 |
| Mgi Id | MGI:3701196 | Doi | 10.4049/jimmunol.175.8.5043 |
| Citation | Brehm MA, et al. (2005) Rapid production of TNF-alpha following TCR engagement of naive CD8 T cells. J Immunol 175(8):5043-9 |
| abstractText | The acquisition of effector functions by naive CD8 T cells following TCR engagement is thought to occur sequentially with full functionality being gained only after the initiation of division. We show that naive CD8 T cells are capable of immediate effector function following TCR engagement, which stimulates the rapid production of TNF-alpha. Stimulation of splenocytes from naive mice of differing genetic backgrounds with anti-CD3epsilon mAb resulted in significant production of TNF-alpha by naive CD8 T cells within 5 h. Moreover, naive lymphocytic choriomeningitis virus-specific TCR-transgenic CD8 T cells stimulated with either their cognate peptide ligand or virus-infected cells produced TNF-alpha as early as 2 h poststimulation, with production peaking by 4 h. Naive CD8 T cells produced both membrane-bound and soluble TNF-alpha. Interfering with TNF-alpha activity during the initial encounter between naive CD8 T cells and Ag loaded dendritic cells altered the maturation profile of the APC and diminished the overall viability of the APC population. These findings suggest that production of TNF-alpha by naive CD8 T cells immediately after TCR engagement may have an unappreciated impact within the local environment where Ag presentation is occurring and potentially influence the development of immune responses. |
