| First Author | Nakanishi T | Year | 1999 |
| Journal | FEBS Lett | Volume | 449 |
| Issue | 2-3 | Pages | 277-83 |
| PubMed ID | 10338148 | Mgi Jnum | J:97291 |
| Mgi Id | MGI:3575105 | Doi | 10.1016/s0014-5793(99)00433-0 |
| Citation | Nakanishi T, et al. (1999) Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein. FEBS Lett 449(2-3):277-83 |
| abstractText | We produced transgenic mouse lines that accumulate mutated green fluorescent protein (EGFP) in sperm acrosome, a membrane limited organelle overlying the nucleus. The sperm showed normal fertilizing ability and the integrity of their acrosome was easily examined in a non-invasive manner by tracing the GFP in individual 'live' sperm with fluorescent microscopy. The time required for the dispersal of acrosomal contents was demonstrated to be approximately 3 s after the onset of acrosome reaction. |
