| First Author | Le Gallou S | Year | 2018 |
| Journal | J Exp Med | Volume | 215 |
| Issue | 8 | Pages | 2035-2053 |
| PubMed ID | 29959173 | Mgi Jnum | J:265925 |
| Mgi Id | MGI:6201768 | Doi | 10.1084/jem.20180977 |
| Citation | Le Gallou S, et al. (2018) A splenic IgM memory subset with antibacterial specificities is sustained from persistent mucosal responses. J Exp Med 215(8):2035-2053 |
| abstractText | To what extent immune responses against the gut flora are compartmentalized within mucosal tissues in homeostatic conditions remains a much-debated issue. We describe here, based on an inducible AID fate-mapping mouse model, that systemic memory B cell subsets, including mainly IgM(+) B cells in spleen, together with IgA(+) plasma cells in spleen and bone marrow, are generated in mice in the absence of deliberate immunization. While the IgA component appears dependent on the gut flora, IgM memory B cells are still generated in germ-free mice, albeit to a reduced extent. Clonal relationships and renewal kinetics after anti-CD20 treatment reveal that this long-lasting splenic population is mainly sustained by output of B cell clones persisting in mucosal germinal centers. IgM-secreting hybridomas established from splenic IgM memory B cells showed reactivity against various bacterial isolates and endogenous retroviruses. Ongoing activation of B cells in gut-associated lymphoid tissues thus generates a diversified systemic compartment showing long-lasting clonal persistence and protective capacity against systemic bacterial infections. |
