| First Author | Martin HM | Year | 1995 |
| Journal | Gene | Volume | 159 |
| Issue | 2 | Pages | 187-91 |
| PubMed ID | 7622047 | Mgi Jnum | J:26842 |
| Mgi Id | MGI:74272 | Doi | 10.1016/0378-1119(95)00123-n |
| Citation | Martin HM, et al. (1995) Cloning and characterisation of an ovine interleukin-10-encoding cDNA. Gene 159(2):187-91 |
| abstractText | Expression of the interleukin 10-encoding (IL-10) mRNA by ovine (ov-) cells, in response to mitogenic stimulation, was assessed by Northern blot and polymerase chain reaction (PCR) analyses using a human (hu) IL-10 cDNA probe and oligodeoxyribonucleotide primers based on homologous regions of the human and murine IL-10 cDNA sequences. A 315-bp cDNA generated by the PCR analysis was cloned and used to screen a lipopolysaccharide-stimulated alveolar ov-macrophage cDNA library. The full-length ov-cDNA sequence isolated translates to a protein of 177 amino acids (aa) with a predicted 18-aa leader sequence and molecular mass of 20,165 Da. Expression in a mammalian system demonstrated that the ov-cDNA encoded a protein with the expected IL-10 biological activity. Both recombinant huIL-10 and supernatants from COS cells transfected with an expression vector containing the ovIL-10 cDNA inhibited production of IL-1 and tumour necrosis factor-alpha by ov-alveolar macrophages. Genomic DNA analysis indicated ovIL-10 exists as a single gene within the ov-genome. |
