| First Author | Fisher EM | Year | 1985 |
| Journal | Proc Natl Acad Sci U S A | Volume | 82 |
| Issue | 17 | Pages | 5846-9 |
| PubMed ID | 3862102 | Mgi Jnum | J:8003 |
| Mgi Id | MGI:56472 | Doi | 10.1073/pnas.82.17.5846 |
| Citation | Fisher EM, et al. (1985) Microdissection and microcloning of the mouse X chromosome. Proc Natl Acad Sci U S A 82(17):5846-9 |
| abstractText | A wild mouse (CD) karyotype in which all the chromosomes bar the X, 19, and Y, are fused as metacentrics has been used for the microdissection and microcloning of a specific mouse X chromosome region. Dissection of a proximal region of the X chromosome encompassing the genetic loci Hprt to Tfm and including mdx has yielded 650 clones. A number of the recovered clones containing sizable inserts have been confirmed as X chromosome specific. This X chromosome bank of clones provides a start point for the isolation of the mdx locus. It is now clear that microdissection and microcloning can be applied to all mouse chromosomes, including the X chromosome, yielding premapped banks of clones that will greatly aid in the isolation and characterization of important genetic loci. |
