| First Author | Jones IM | Year | 1987 |
| Journal | Somat Cell Mol Genet | Volume | 13 |
| Issue | 4 | Pages | 325-33 |
| PubMed ID | 2842875 | Mgi Jnum | J:20242 |
| Mgi Id | MGI:68350 | Doi | 10.1007/BF01534926 |
| Citation | Jones IM, et al. (1987) Cloned mouse lymphocytes permit analysis of somatic mutations that occur in vivo. Somat Cell Mol Genet 13(4):325-33 |
| abstractText | As part of our mouse model of somatic mutation, we have begun to characterize spontaneously occurring hypoxanthine phosphoribosyltransferase (HPRT) -deficient mouse lymphocytes. Lymphocytes were cloned by in vitro exposure of spleen cells from male C57B1/6 mice to the mitogen concanavalin A, conditioned medium containing lymphocyte growth factors, and thioguanine (TG), in a limiting dilution assay. The 17 TG-resistant clones recovered were all highly deficient in HPRT activity and were found by analysis of surface antigens to be representative of the major subclasses of T lymphocytes. Southern analysis of lymphocyte genomic DNA detected alterations of the hprt gene in 12/17 of the HPRT-deficient lymphocyte clones. Of these 12, 2/17 were lacking the entire hprt locus, 7/17 lacked part of the locus, and 3/17 had other, unidentified alterations. |
