| First Author | Sopjani M | Year | 2011 |
| Journal | FEBS Lett | Volume | 585 |
| Issue | 12 | Pages | 1759-64 |
| PubMed ID | 21605558 | Mgi Jnum | J:173340 |
| Mgi Id | MGI:5013881 | Doi | 10.1016/j.febslet.2011.05.021 |
| Citation | Sopjani M, et al. (2011) Regulation of the na(+)/k(+) ATPase by klotho. FEBS Lett 585(12):1759-64 |
| abstractText | Klotho-hypomorphic (Klotho(hm)) mice suffer from renal salt wasting and hypovolemia despite hyperaldosteronism. The present study explored the effect of Klotho on renal Na(+)/K(+) ATPase activity. According to immunohistochemistry and confocal microscopy Na(+)/K(+) ATPase protein abundance in isolated collecting ducts was lower in Klotho(hm) mice than in their wild type littermates (Klotho(+/+)). Analysis with dual electrode voltage clamp recording showed that expression of Klotho in Xenopus oocytes increased the Na(+)/K(+) ATPase pump current. Treatment of Xenopus oocytes with Klotho protein similarly increased the pump current. In conclusion, Klotho increases the membrane abundance and activity of the Na(+)/K(+) ATPase. Decreased Na(+)/K(+) ATPase activity could thus contribute to the volume-depletion of klotho(hm) mice. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: Na+/K+ ATPase alpha-1physically interacts with Klotho by anti bait coimmunoprecipitation (View interaction). |
