| First Author | Deato MD | Year | 2008 |
| Journal | Mol Cell | Volume | 32 |
| Issue | 1 | Pages | 96-105 |
| PubMed ID | 18851836 | Mgi Jnum | J:141107 |
| Mgi Id | MGI:3815378 | Doi | 10.1016/j.molcel.2008.09.009 |
| Citation | Deato MD, et al. (2008) MyoD targets TAF3/TRF3 to activate myogenin transcription. Mol Cell 32(1):96-105 |
| abstractText | Skeletal muscle differentiation requires a cascade of transcriptional events to control the spatial and temporal expression of muscle-specific genes. Until recently, muscle-specific transcription was primarily attributed to prototypic enhancer-binding factors, while the role of core promoter recognition complexes in directing myogenesis remained unknown. Here, we report the development of a purified reconstituted system to analyze the properties of a TAF3/TRF3 complex in directing transcription initiation at the Myogenin promoter. Importantly, this new complex is required to replace the canonical TFIID to recapitulate MyoD-dependent activation of Myogenin. In vitro and cell-based assays identify a domain of TAF3 that mediates coactivator functions targeted by MyoD. Our findings also suggest changes to CRSP/Mediator in terminally differentiated myotubes. This switching of the core promoter recognition complex during myogenesis allows a more balanced division of labor between activators and TAF coactivators, thus providing another strategy to accommodate cell-specific regulation during metazoan development. |
