| First Author | Hoyer-Fender S | Year | 1998 |
| Journal | Mol Reprod Dev | Volume | 51 |
| Issue | 2 | Pages | 167-75 |
| PubMed ID | 9740324 | Mgi Jnum | J:49572 |
| Mgi Id | MGI:1277712 | Doi | 10.1002/(SICI)1098-2795(199810)51:2<167::AID-MRD6>3.0.CO;2-O |
| Citation | Hoyer-Fender S, et al. (1998) Mouse Odf2 cDNAs consist of evolutionary conserved as well as highly variable sequences and encode outer dense fiber proteins of the sperm tail. Mol Reprod Dev 51(2):167-75 |
| abstractText | The outer dense fibers (ODF) of the mammalian sperm tail comprise a unique, specialized, and very prominent structure, consisting of nine fibers surrounding the axoneme. The ODF may play an important but as yet undefined role in sperm morphology, integrity and function. Study of the ODF is hampered by insufficient knowledge of their protein composition and the genetic regulation of their synthesis. We report here on the characterization of cDNAs encoding the Odf2 proteins of outer dense fibers of mouse sperm. We isolated two cDNA clones with variable 5' regions. Variability in sequence is restricted to specific regions in the N-terminal part of the encoded proteins, whereas the C- terminal part is highly conserved in Odf2 proteins both between species and within a species. This variability is confirmed at the protein level. The outer dense fibers could be detected immunologically in total sperm tails allowing a direct comparison of their length in relation to the length of the sperm tail. Odf2 transcripts could be demonstrated in testicular RNA and are restricted to germ cells. The start of transcription is in step 5 spermatids of tubular stage V and the RNA could be detected in the cytoplasm of differentiating spermatids in all subsequent tubular stages. |
