| First Author | Nandiwada SL | Year | 2006 |
| Journal | J Immunol | Volume | 177 |
| Issue | 1 | Pages | 401-13 |
| PubMed ID | 16785536 | Mgi Jnum | J:134401 |
| Mgi Id | MGI:3785652 | Doi | 10.4049/jimmunol.177.1.401 |
| Citation | Nandiwada SL, et al. (2006) p300/Cyclic AMP-responsive element binding-binding protein mediates transcriptional coactivation by the CD28 T cell costimulatory receptor. J Immunol 177(1):401-13 |
| abstractText | During Ag stimulation of T cells, the recognition of B7 molecules by the CD28 costimulatory receptor increases the level of c-Fos, a component of the AP-1 transactivator known to bind the 5' Il2 gene enhancer. In this study, we show that the costimulation of Fos transcription by CD28 is associated with increased binding of p300/CREB-binding protein (CBP) molecules at the Fos promoter, and is blocked by an adenoviral E1A molecular antagonist of p300/CBP. Furthermore, transcriptional activation by a C-terminal domain of CBP is strengthened when CD28 molecules are actively signaling. This increased amount and activity of p300/CBP molecules at the Fos gene correlated with higher histone H4 acetylation and RNA polymerase II association with the promoter. These data suggest a global mechanism whereby CD28 signaling influences the rate and intensity of new gene expression during Ag recognition via direct control over the coactivator function of p300/CBP. |
