| Primary Identifier | MGI:5911847 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Slc22a6 |
| Transmission | Germline | Strain of Origin | C57BL/6J |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| description | C57BL/6J embryonic stem cells (CRIB6.1, an ES line derived in house) were used. |
| molecularNote | Using recombineering methods, the CreERT2-Frt-PGK-Hygromycin-pA-Frt cassette replaced the ATG start codon in exon 1 of the Slc22a6 gene in the BAC clone RP23-457i11. The modified BAC construct was electroporated into ES cells and correctly targeted ES cells were injected into CD1 blastocysts and the resulting chimeric mice were bred to C57BL/6J mice. These mice were then bred to mice containing Flp-recombinase to remove the Frt-flanked hygromycin selection cassette. |
