Primary Identifier | MGI:6436701 | Allele Type | Endonuclease-mediated |
Attribute String | Not Specified | Gene | Gcn1 |
Strain of Origin | C57BL/6 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 technology was used to induce double-strand breaks within introns 45 and 53 and excise exons 46-53, thus deleting the RWD Binding Domain (RWDBD). Immunoblot analysis of homozygous mutant mouse embryonic fibroblasts (MEFs) revealed that the level of protein expression is reduced to 30% relative to that in wild-type MEFs. |