| First Author | Gangeswaran R | Year | 1997 |
| Journal | FEBS Lett | Volume | 412 |
| Issue | 2 | Pages | 309-12 |
| PubMed ID | 9256241 | Mgi Jnum | J:42135 |
| Mgi Id | MGI:1095221 | Doi | 10.1016/s0014-5793(97)00782-5 |
| Citation | Gangeswaran R, et al. (1997) Unique protein kinase C profile in mouse oocytes: lack of calcium-dependent conventional isoforms suggested by rtPCR and Western blotting. FEBS Lett 412(2):309-12 |
| abstractText | rtPCR and Western blotting were used to determine which members of the PKC family are present in both immature and mature mouse oocytes. Using isoform-specific PCR primers and antibodies PKC-delta and -lambda were detected while such techniques failed to observe the conventional isoforms of PKC-alpha, -beta, -gamma. This isoform profile was confirmed using an alternative PCR strategy, which allowed discrimination of PCR products derived from conventional and novel PKC isoforms. In addition PKC-epsilon, -eta, -theta and -zeta were not detected by rtPCR. These results suggest that the predominant isoforms in oocytes are PKC-delta and -lambda. |
