| First Author | Mohri Y | Year | 2011 |
| Journal | Dev Dyn | Volume | 240 |
| Issue | 6 | Pages | 1626-34 |
| PubMed ID | 21523854 | Mgi Jnum | J:172277 |
| Mgi Id | MGI:5006869 | Doi | 10.1002/dvdy.22651 |
| Citation | Mohri Y, et al. (2011) Lgr4-deficient mice showed premature differentiation of ureteric bud with reduced expression of Wnt effector Lef1 and Gata3. Dev Dyn 240(6):1626-34 |
| abstractText | We previously reported that Lgr4 has a critical role in the morphogenesis of kidney, but the detailed functions of Lgr4 in kidney development have not been elucidated. In contrast to Lgr4 null mice with 129Ola x C57BL/6J mixed background, C57BL/6J-backcrossed Lgr4 null mice (Lgr4(-/-) ) showed the severe phenotype of embryonic lethality and also had dilated tubules in kidneys at E16.5. Based on quantitative RT-PCR and in situ hybridization, branching morphogenesis at E15.5 in the Lgr4(-/-) was arrested earlier, and both DBA-lectin staining and immunohistochemical analysis using Aqp3 antibodies showed that the ureteric bud (UB) of Lgr4(-/-) kidneys underwent premature differentiation. Furthermore, quantitative RT-PCR and histological analysis suggested that the impaired UB differentiation was caused by down-regulation of the Wnt pathway and Gata3 in the Lgr4(-/-) kidneys. We demonstrate here that Lgr4 has a novel function for maintaining the UB in an undifferentiated state. Developmental Dynamics 240:1626-1634, 2011. (c) 2011 Wiley-Liss, Inc. |
