| First Author | Matsuura A | Year | 1994 |
| Journal | Cell Immunol | Volume | 155 |
| Issue | 2 | Pages | 501-7 |
| PubMed ID | 8181080 | Mgi Jnum | J:37904 |
| Mgi Id | MGI:85300 | Doi | 10.1006/cimm.1994.1142 |
| Citation | Matsuura A, et al. (1994) Cyclophosphamide-induced tolerance in fully allogeneic heart transplantation in mice. Cell Immunol 155(2):501-7 |
| abstractText | We have established a long-lasting heart allograft tolerance in H-2-incompatible combinations by intravenous injection of 10(8) allogeneic spleen cells followed by the administration of 200 mg/kg of cyclophosphamide (CP). When C57BL/10 mice (B10, H-2b) were primed iv with 1 x 10(8) viable spleen cells from naive BALB/c mice (H-2d) and treated ip with 200 mg/kg CP 2 days later, BALB/c heart allografts were lasting in the recipients for as long as 8 weeks. On the other hand, B10 mice receiving either allogeneic spleen cells or CP rejected the heart allograft within 10 days after transplantation. A direct approach assessing the donor-reactive T cells for frequency and reactivity showed that the donor-reactive T cells were partially deleted and the rest were rendered unresponsive. CP-induced tolerance may be applied for the induction of antigen-specific tolerance to allogeneic heart transplantation. |
