| Primary Identifier | MGI:2675502 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Dnase2b |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 3 and 4 were replaced with a neomycin selection cassette inserted by homologous recombination. Included in the deleted region were codons for 2 conserved histidine residues that are putative enzymatic active sites. Truncated transcript lacking the targeted sequence, and consequently containing a frameshift mutation, was detected in mutant mice by both Northern and RT-PCR analyses. Absence of DNAse activity under acidic conditions indicated that stable and functional protein was not produced by the mutant transcript. |
