| First Author | Ogura T | Year | 1993 |
| Journal | Biochem Biophys Res Commun | Volume | 193 |
| Issue | 3 | Pages | 1014-22 |
| PubMed ID | 7686743 | Mgi Jnum | J:39582 |
| Mgi Id | MGI:86936 | Doi | 10.1006/bbrc.1993.1726 |
| Citation | Ogura T, et al. (1993) Structural diversity of neuronal nitric oxide synthase mRNA in the nervous system. Biochem Biophys Res Commun 193(3):1014-22 |
| abstractText | Nitric oxide (NO), generated from L-arginine by an enzymatic reaction of a NO synthase (NOS; EC 1.14.23), is a recently identified biological mediator suggested to be involved in a wide variety of biological processes. In the present work, we isolated and sequenced the entire region of cDNAs encoding mouse neuronal NOS (n-NOS) and demonstrated structural diversity of n-NOS mRNA in the nervous system. Sequence determination revealed a novel mRNA with an inframe deletion in the middle of the n-NOS cDNA. Structural analysis of the corresponding part of the n-NOS gene indicated that the deletion corresponded exactly to two exons. These findings suggest that the variant n-NOS is formed by alternative splicing. Both n-NOSs were found in almost all parts of the nervous system, the expression level of the novel variant being about one twentieth of that of the known n-NOS. Generation of diversity of n-NOS is probably related to diversity of its biological functions. |
