| First Author | Kawasaki H | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 3 | Pages | e58221 |
| PubMed ID | 23505471 | Mgi Jnum | J:322660 |
| Mgi Id | MGI:6234191 | Doi | 10.1371/journal.pone.0058221 |
| Citation | Kawasaki H, et al. (2013) The circadian binding of CLOCK protein to the promoter of C/ebpalpha gene in mouse cells. PLoS One 8(3):e58221 |
| abstractText | C/EBPalpha plays important roles in metabolism as well as in the maintenance of energy homeostasis. Here we describe loss of the circadian oscillation of C/ebpalpha expression in liver of Clock mutant mice. Reporter assays indicate Clock and Bmal significantly induced C/ebpalpha gene expression whereas Cry suppressed. Real time reporter assays showed that two mutated E-boxes disrupted C/ebpalpha promoter dependent-oscillation. Chromatin immunoprecipitation suggests Clock can bind to two E-boxes in the C/ebpalpha promoter with a circadian manner in vivo. Thus, C/ebpalpha gene transcription is under circadian control of a core clock component, Clock. The data suggests that circadian disturbances may affect metabolic abnormalities through the C/ebpalpha pathway in liver. |
