| First Author | Phillips SC | Year | 1992 |
| Journal | Gene | Volume | 116 |
| Issue | 2 | Pages | 181-6 |
| PubMed ID | 1634116 | Mgi Jnum | J:1504 |
| Mgi Id | MGI:50031 | Doi | 10.1016/0378-1119(92)90514-p |
| Citation | Phillips SC, et al. (1992) A transcriptional analysis of the gene encoding mouse U7 small nuclear RNA. Gene 116(2):181-6 |
| abstractText | Expression of the U7 gene, encoding mouse U7 snRNA, following microinjection into Xenopus oocytes is both accurate and efficient, giving rise to mature U7 snRNA and a precursor with an 8-nucleotide (nt) 3' extension. The mouse U7 gene promoter, which is similar to that of the vertebrate major U genes comprising a DSE, a PSE and a 3' box, with the same spatial arrangement, is as efficient as the Xenopus U2 gene promoter in this assay. A deletion analysis of the mouse U7 gene identified sequences downstream from the 3' box, within the region (nt +74 to +196), which seem to have a negative regulatory effect upon the frequency of transcription initiation and are also required for accurate 3' end formation. Sequences in the nt -1699 to -431 region also seemed to have a negative effect on the level of transcription. In addition, sequences upstream from the PSE, within the nt -65 to -421 region, are necessary for accurate and efficient synthesis of mature U7 snRNA. Finally, the mouse U7 snRNA may not form a functional snRNP in Xenopus oocytes due to defective snRNP assembly and/or nuclear import. |
