| First Author | Song J | Year | 2004 |
| Journal | J Biol Chem | Volume | 279 |
| Issue | 23 | Pages | 24414-9 |
| PubMed ID | 15047706 | Mgi Jnum | J:90741 |
| Mgi Id | MGI:3044526 | Doi | 10.1074/jbc.M311406200 |
| Citation | Song J, et al. (2004) Cyclophilin A is required for retinoic acid-induced neuronal differentiation in p19 cells. J Biol Chem 279(23):24414-9 |
| abstractText | Stable transfectants with expression of small interfering RNA for targeting cyclophilin A (CypA) in p19 cells lose their potential for retinoic acid (RA)-induced neuronal differentiation but not Me(2)SO-induced mesodermal differentiation. This difference suggests that CypA is specifically required for the RA-induced neuronal pathway. In addition to the loss of RA-induced RA receptor beta expression and retinoic acid response element (RARE)-binding activity, a dramatic reduction in RA-induced RARE-mediated luciferase activity in the CypA knockdown cell line suggests that CypA affects RARE-mediated regulation of gene expression. Silent mutation of target sequences confirms the specificity of RNA interference in p19 embryonal carcinoma cells. Collectively, our data reveal that a novel function of CypA is required in the processing of RA-induced neuronal differentiation in p19 embryonal carcinoma cells. |
