Primary Identifier | MGI:2182179 | Allele Type | Targeted |
Attribute String | Inserted expressed sequence, Null/knockout | Gene | Mesp2 |
Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A construct containing a downstream neomycin resistance gene and Mesp1 cDNA containing the entire coding region positioned at the start site of the Mesp2 gene was inserted into the Mesp2 locus. A non-coding region of Mesp2 was left intact and utilized for RT-PCR analysis, which showed that transcript from the Mesp2 locus was absent, while transcript from both the endogenous and transgenic Mesp1 loci were present. |