Primary Identifier | MGI:6715680 | Allele Type | Endonuclease-mediated |
Gene | Tmc1 | Strain of Origin | C57BL/6J |
Is Recombinase | false | Is Wild Type | false |
molecularNote | CRISPR/cas9 genome editing is used to introduce a W554L (TGG to CTG) mutation into exon 15 to modify residues near the putative pore of the channel. Three additional silent mutations were also created to add a BstAP1 enzyme restriction site. |