Primary Identifier | MGI:6712742 | Allele Type | Targeted |
Attribute String | Epitope tag, Reporter | Gene | Mesp1 |
Transmission | Germline | Strain of Origin | 129S/SvEv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting construct introduced replaced the first 337 bp of the coding sequence in exon 1 a membrane-targeted Venus reporter gene (myr-Venus), a T2A sequence, the coding sequence of the endogenous gene, three FLAG epitope tags, and polyadenylation sequence (bGH PolyA). Cre-mediated recombination removed the floxed neomycin selection cassette introduced downstream of the inserted sequence. |