| Primary Identifier | MGI:4397586 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Gtsf1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:154374 does not mention whether the null allele of Pou5f1 is also present in the line of mice created. |
| molecularNote | Exons 2 through 4 were replaced with an IRES-beta-geo and puro cassette. The absence of protein expression was confirmed by western blot analysis on week 7 testis extracts. |
