| Primary Identifier | MGI:2182450 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Dio2 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neomycin selection cassette replaced a 2.6kb region including a 5' portion of exon 2. 72% of the coding region was deleted, including a TGA codon that encodes the selenocysteine located at the active site. Northern blot analysis of total RNA obtained from brown adipose tissue showed an absence of transcript in homozygous mutant mice. Functional ablation of the endogenous protein in homozygous mutant mice was confirmed by assaying deiodination in brown adipose tissue. |
