| First Author | Cone RE | Year | 1978 |
| Journal | J Immunol | Volume | 120 |
| Issue | 5 | Pages | 1733-40 |
| PubMed ID | 307021 | Mgi Jnum | J:5989 |
| Mgi Id | MGI:54466 | Doi | 10.4049/jimmunol.120.5.1733 |
| Citation | Cone RE, et al. (1978) Molecular identification of a surface structure on B cells (Lyb-3) and its relationship to B cell triggering. J Immunol 120(5):1733-40 |
| abstractText | Lactoperoxidase-catalyzed radioiodination of cell surface proteins and immunochemical procedures are used to identify murine splenic lymphocyte membrane components bound by anti-Lyb-3 serum. This antiserum defines membrane components (Lyb-3) on a subpopulation of murine B cells that may function as a receptor for T cell signals. SDS-PAGE analysis of surface-labeled membrane components bound by anti-Lyb-3 serum demonstrated a single molecular species of 68,000 d. The polypeptides recognized by anti-Lyb-3 are not composed of disulfide-linked subunits and bear no antigenic relationship with known membrane immunoglobulins (IgM or IgD). Absorption of anti-Lyb-3 serum with the 68,000 d polypeptides removed the ability of anti-Lyb-3 serum to augment the in vivo immune response of mice to low doses of sheep erythrocytes. The latter provides formal proof that the 68,000 d polypeptide bound by anti-Lyb-3 serum is the target on the B cell membrane for the immunoenhancing activity of the antiserum. |
