Primary Identifier | MGI:5660892 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Hbb-b2 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A floxed neo cassette replaced 600 bp of the promoter region, up to the first 25 bp of exon 1 (+43653-44287), via homologous recombination. Cre mediated recombination removed the neo cassette. Quantitative RT-PCR confirmed the absence of expression in peripheral blood and fetal liver from homozygous mice. |