| First Author | Chiang W | Year | 2000 |
| Journal | Dev Dyn | Volume | 217 |
| Issue | 1 | Pages | 99-108 |
| PubMed ID | 10679933 | Mgi Jnum | J:59239 |
| Mgi Id | MGI:1351230 | Doi | 10.1002/(SICI)1097-0177(200001)217:1<99::AID-DVDY9>3.0.CO;2-5 |
| Citation | Chiang W, et al. (2000) Filamin isogene expression during mouse myogenesis. Dev Dyn 217(1):99-108 |
| abstractText | The developmental pattern of filamin gene expression has been studied in mouse embryos by using in situ hybridization. The probes used were isoform specific, (35)S-labeled antisense complementary ribonucleic acids (cRNAs) to the 3; untranslated region (3; UTR) of muscle-specific and nonmuscle-specific filamin genes. Northern blot and in situ hybridization results showed that nonmuscle-specific filamin transcripts had a size of 9.5 kb and were expressed in all nonmuscle tissues. Labeling was most intense in tissues containing a substantial proportion of epithelial and smooth muscle cells. Muscle-specific filamin transcripts had a size of 10 kb and were expressed primarily in cardiac and skeletal muscle. The expression of muscle-specific filamin messenger ribonucleicacids (mRNAs) was detected in heart at 8.0 days after coitum, whereas that in the myotomes of somites was not detected until 10.5 days after coitum. The expression of muscle-specific filamin mRNAs in heart and in skeletal muscle continued through the subsequent days of myogenesis. The results showed that muscle-specific filamin gene transcripts are detected before the formation of myotubes in vivo. This is the first study of filamin gene expression at the early stages of skeletal muscle development. Dev Dyn 2000;217:99-108. Copyright 2000 Wiley-Liss, Inc. |
