| First Author | Lu P | Year | 2023 |
| Journal | Dev Cell | Volume | 58 |
| Issue | 9 | Pages | 791-805.e4 |
| PubMed ID | 37023750 | Mgi Jnum | J:335167 |
| Mgi Id | MGI:7468545 | Doi | 10.1016/j.devcel.2023.03.009 |
| Citation | Lu P, et al. (2023) Prerequisite endocardial-mesenchymal transition for murine cardiac trabecular angiogenesis. Dev Cell |
| abstractText | Coronary heart disease damages the trabecular myocardium, and the regeneration of trabecular vessels may alleviate ischemic injury. However, the origins and developmental mechanisms of trabecular vessels remain unknown. Here, we show that murine ventricular endocardial cells generate trabecular vessels through an "angioEMT" mechanism. Time course fate mapping defined a specific wave of trabecular vascularization by ventricular endocardial cells. Single-cell transcriptomics and immunofluorescence identified a subpopulation of ventricular endocardial cells that underwent endocardial-mesenchymal transition (EMT) before these cells generated trabecular vessels. Ex vivo pharmacological activation and in vivo genetic inactivation experiments identified an EMT signal in ventricular endocardial cells involving SNAI2-TGFB2/TGFBR3, which was a prerequisite for later trabecular-vessel formation. Additional loss- and gain-of-function genetic studies showed that VEGFA-NOTCH1 signaling regulated post-EMT trabecular angiogenesis by ventricular endocardial cells. Our finding that trabecular vessels originate from ventricular endocardial cells through a two-step angioEMT mechanism could inform better regeneration medicine for coronary heart disease. |
