| First Author | Pindon A | Year | 1997 |
| Journal | Glia | Volume | 19 |
| Issue | 3 | Pages | 259-68 |
| PubMed ID | 9063732 | Mgi Jnum | J:39181 |
| Mgi Id | MGI:86565 | Citation | Pindon A, et al. (1997) Novel expression and localization of active thrombomodulin on the surface of mouse brain astrocytes. Glia 19(3):259-68 |
| abstractText | Thrombin's potent effects on astrocytes are mediated by a specific receptor and inhibited by a serpin, protease nexin I (PNI). Thrombomodulin (TM), a membrane protein that forms complexes with thrombin, changing its enzymatic specificity, has not been studied in astrocytes. In primary astrocyte cultures, using Western blotting and immunocytochemistry, we found a 70 kDa TM band and TM localized to the surface with an anti-mouse TM monoclonal antibody. By reverse transcriptase coupled with polymerase chain reaction (RT-PCR), we found the correct sequence for mouse TM mRNA in astrocytes. Finally, we documented calcium-dependent activation of protein C by a thrombin:TM complex with thrombin added to the astrocytes. These results indicate the presence of functionally active TM at the astrocyte surface and add support to a role for thrombin signaling in the nervous system. |
