| First Author | Momotani K | Year | 2008 |
| Journal | Biochem J | Volume | 412 |
| Issue | 2 | Pages | 265-73 |
| PubMed ID | 18294141 | Mgi Jnum | J:137954 |
| Mgi Id | MGI:3803436 | Doi | 10.1042/BJ20071501 |
| Citation | Momotani K, et al. (2008) Cep57, a multidomain protein with unique microtubule and centrosomal localization domains. Biochem J 412(2):265-73 |
| abstractText | The present study demonstrates different functional domains of a recently described centrosomal protein, Cep57 (centrosomal protein 57). Endogenous Cep57 protein and ectopic expression of full-length protein or the N-terminal coiled-coil domain localize to the centrosome internal to gamma-tubulin, suggesting that it is either on both centrioles or on a centromatrix component. The N-terminus can also multimerize with the N-terminus of other Cep57 molecules. The C-terminus contains a second coiled-coil domain that directly binds to MTs (microtubules). This domain both nucleates and bundles MTs in vitro. This activity was also seen in vivo, as overexpression of full-length Cep57 or the C-terminus generates nocodazole-resistant MT cables in cells. Based on the present findings, we propose that Cep57 serves as a link with its N-terminus anchored to the centriole or centromatrix and its C-terminus to MTs. |
