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Allele : Scgb1a1<tm2.1(CtTA)Thbr> secretoglobin, family 1A, member 1; targeted mutation 2.1, Thomas Braun

Primary Identifier  MGI:7263086 Allele Type  Targeted
Attribute String  Epitope tag, Reporter, Transactivator Gene  Scgb1a1
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Is Recombinase  false Is Wild Type  false
molecularNote  Mice were generated by inserting a 2A sequence, mCherry, 2A sequence, and the C terminal 36 amino acids of the DnaE split intein sequence from Nostoc punctiforme (Npu) fused to the C-terminal fragment of tTA (aa 206-248), FLAG tag, and Frt-flanked neomycin resistance cassette immediately in front of the stop codon of Scgb1a1 coding sequence by homologous recombination. The Frt-flanked neomycin resistance cassette was removed by crossing with hACTB-Flpe deleter mice.
  • mutations:
  • Insertion
  • synonyms:
  • CCSP<-2A-mCherry-2A-tTA-C>,
  • CCSP<-2A-mCherry-2A-tTA-C>
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

0 Driven By

3 Publication categories

Trail: Allele