Primary Identifier | MGI:6507764 | Allele Type | Endonuclease-mediated |
Attribute String | Conditional ready, No functional change | Gene | Dhx15 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | LoxP sites were inserted upstream of exon 1 and into intron 3 using two gRNAs (targeting TGAACTGCAGCAGCGTTCCT and AGATATTATTAGAGTAGCCG) and two ssODN templates with CRISPR/Cas9 technology, creating a conditional-ready allele with exons 1-3 floxed. |