Primary Identifier | MGI:6448430 | Allele Type | Endonuclease-mediated |
Attribute String | Epitope tag | Gene | Tlr9 |
Strain of Origin | C57BL/6J | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The sequence just upstream of the stop codon was targeted for insertion of HA tag sequence with a gRNA (5'-GGGACCTACAGCAGAATAG-3') and an oligonucleotide donor, using CRISPR/Cas9 technology. The resulting allele has sequence coding for nine C-terminal haemagglutinin (HA) amino-acids inserted in-frame immediately upstream of the stop codon. |