| Primary Identifier | MGI:3831175 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A cassette containing a splice acceptor, the puromycin resistance gene, and a poly A sequence was placed in front of a CAGGS promoter. Downstream of the promoter was placed a floxed terminator element containing three SV40 poly A sequences (tpA) followed by a bicistronic cassette encoding for Wnt7a and IRES-nlacZ. In the absence of Cre recombinase, transcription from the CAGGS enhancer/promoter is terminated by the tpA element. In the presence of cre recombinase, the tpA is recombined out and the CAGGS enhancer/promoter drives expression of both Wnt7a and betagalactosidase. Ectopic Wnt7a and lacZ expression were detected in the neuroepithelium of developing embryos when this allele was on a Tg(Nes-cre)1Kln transgenic background. |
