| First Author | Reed J | Year | 2019 |
| Journal | J Immunol | Volume | 202 |
| Issue | 10 | Pages | 2873-2887 |
| PubMed ID | 30962293 | Mgi Jnum | J:274890 |
| Mgi Id | MGI:6296837 | Doi | 10.4049/jimmunol.1801577 |
| Citation | Reed J, et al. (2019) Trogocytosis-Mediated Intracellular Signaling in CD4(+) T Cells Drives TH2-Associated Effector Cytokine Production and Differentiation. J Immunol 202(10):2873-2887 |
| abstractText | CD4(+) T cells have been observed to acquire APC-derived membrane and membrane-associated molecules through trogocytosis in diverse immune settings. Despite this, the consequences of trogocytosis on the recipient T cell remain largely unknown. We previously reported that trogocytosed molecules on CD4(+) T cells engage their respective surface receptors, leading to sustained TCR signaling and survival after APC removal. Using peptide-pulsed bone marrow-derived dendritic cells and transfected murine fibroblasts expressing antigenic MHC:peptide complexes as APC, we show that trogocytosis-positive CD4(+) T cells display effector cytokines and transcription factor expression consistent with a TH2 phenotype. In vitro-polarized TH2 cells were found to be more efficient at performing trogocytosis than TH1 or nonpolarized CD4(+) cells, whereas subsequent trogocytosis-mediated signaling induced TH2 differentiation in polarized TH1 and nonpolarized cells. Trogocytosis-positive CD4(+) T cells generated in vivo also display a TH2 phenotype in both TCR-transgenic and wild-type models. These findings suggest that trogocytosis-mediated signaling impacts CD4(+) T cell differentiation and effector cytokine production and may play a role in augmenting or shaping a TH2-dominant immune response. |
