| First Author | Zhang J | Year | 2014 |
| Journal | PLoS One | Volume | 9 |
| Issue | 9 | Pages | e108156 |
| PubMed ID | 25232836 | Mgi Jnum | J:222511 |
| Mgi Id | MGI:5644768 | Doi | 10.1371/journal.pone.0108156 |
| Citation | Zhang J, et al. (2014) The co-stimulatory effects of MyD88-dependent Toll-like receptor signaling on activation of murine gammadelta T cells. PLoS One 9(9):e108156 |
| abstractText | gammadelta T cells express several different toll-like receptor (TLR)s. The role of MyD88- dependent TLR signaling in TCR activation of murine gammadelta T cells is incompletely defined. Here, we report that Pam3CSK4 (PAM, TLR2 agonist) and CL097 (TLR7 agonist), but not lipopolysaccharide (TLR4 agonist), increased CD69 expression and Th1-type cytokine production upon anti-CD3 stimulation of gammadelta T cells from young adult mice (6-to 10-week-old). However, these agonists alone did not induce gammadelta T cell activation. Additionally, we noted that neither PAM nor CL097 synergized with anti-CD3 in inducing CD69 expression on gammadelta T cells of aged mice (21-to 22-month-old). Compared to young gammadelta T cells, PAM and CL097 increased Th-1 type cytokine production with a lower magnitude from anti-CD3- stimulated, aged gammadelta T cells. Vgamma1+ and Vgamma4+ cells are two subpopulations of splenic gammadelta T cells. PAM had similar effects in anti-CD3-activated control and Vgamma4+ subset- depleted gammadelta T cells; whereas CL097 induced more IFN-gamma production from Vgamma4+ subset-depleted gammadelta T cells than from the control group. Finally, we studied the role of MyD88-dependent TLRs in gammadelta T cell activation during West Nile virus (WNV) infection. gammadelta T cell, in particular, Vgamma1+ subset expansion was significantly reduced in both MyD88- and TLR7- deficient mice. Treatment with TLR7 agonist induced more Vgamma1+ cell expansion in wild-type mice during WNV infection. In summary, these results suggest that MyD88-dependent TLRs provide co-stimulatory signals during TCR activation of gammadelta T cells and these have differential effects on distinct subsets. |
