| First Author | Niu K | Year | 2017 |
| Journal | Sci Rep | Volume | 7 |
| Pages | 44809 | PubMed ID | 28333124 |
| Mgi Jnum | J:274469 | Mgi Id | MGI:6296479 |
| Doi | 10.1038/srep44809 | Citation | Niu K, et al. (2017) BAP31 is involved in T cell activation through TCR signal pathways. Sci Rep 7:44809 |
| abstractText | BAP31 is a ubiquitously expressed endoplasmic reticulum (ER) membrane protein. The functions of BAP31 in the immune system have not been investigated due to the lack of animal models. Therefore we created a BAP31 conditional knockdown mouse by performing a knockdown of BAP31 in the thymus. In doing so, we demonstrate that the maturation of T cells is normal but the number of T cells is less in the thymus of the knockout mouse. In addition, the spleen and lymph nodes of peripheral immune organs contained a lesser proportion of the mature T cells in the thymus specific BAP31 knockout mice. The BAP31 knockout T cells decreased the proliferation activated by TCR signal pathways. Further studies clarified that BAP31 affects the phosphorylation levels of both Zap70/Lck/Lat of the upstream members and Akt/GSK/Jnk/Erk of the downstream members of TCR signal pathways. Furthermore, BAP31 can regulate the expression of some markers such as CD3/TCRalpha/TCRbeta and some cytokines like IL-2/IFN-gamma/IL-6/TNF-alpha which are important for T cell activation. Taken together, these results demonstrate that BAP31 may play an important role in T cell activation by regulating TCR signaling. |
