| First Author | Awazu M | Year | 2021 |
| Journal | Biochem Biophys Res Commun | Volume | 559 |
| Pages | 28-34 | PubMed ID | 33932897 |
| Mgi Jnum | J:305526 | Mgi Id | MGI:6705997 |
| Doi | 10.1016/j.bbrc.2021.04.081 | Citation | Awazu M, et al. (2021) Caspase-3 regulates ureteric branching in mice via cell migration. Biochem Biophys Res Commun 559:28-34 |
| abstractText | Inhibition of caspase-3 (Casp3) reduces ureteric branching in organ culture but the mechanism remains unclear. Since Casp3 has non-apoptotic functions, we examined whether Casp3 regulates ureteric branching by promoting cell migration, using a ureteric bud (UB) cell line and Casp3-deficient (Casp3-/-) mice. Also, we examined whether Casp3 plays a role in the reduced ureteric branching of metanephroi from nutrient restricted mothers, in which Casp3 activity is suppressed. A Casp3 inhibitor Ac-DNLD-CHO reduced FGF2-induced cord formation of UB cells in 3D culture. UB cell migration assessed by Boyden chamber and wound healing assays was inhibited by Ac-DNLD-CHO. Glomerular number was reduced by approximately 30%, and ureteric tip number was lower in Casp3-/- mice compared with controls. Maternal nutrient restriction decreased ureteric tip number in controls but not in Casp3-/-. In conclusion, Casp3 regulates ureteric branching by promoting UB cell migration. Inhibited ureteric branching by maternal nutrient restriction may be mediated by Casp3. |
