Primary Identifier | MGI:5699590 | Allele Type | Transgenic |
Attribute String | Inserted expressed sequence | Gene | Tg(Chek1)1Ofc |
Strain of Origin | (C57BL/6 x CBA)F1 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | A 33.5-kb DNA segment containing the mouse checkpoint kinase 1 (Chek1) gene and 5' and 3' flanking sequences extending to the next conserved gene in either direction was subcloned into a minimal vector, which was then linearized for microinjection into fertilized oocytes. Southern blot analysis revealed that one line had integrated a single copy of the transgene. |