| First Author | Jyonouchi H | Year | 1994 |
| Journal | J Nutr | Volume | 124 |
| Issue | 4 | Pages | 475-84 |
| PubMed ID | 8145068 | Mgi Jnum | J:17572 |
| Mgi Id | MGI:65607 | Doi | 10.1093/jn/124.4.475 |
| Citation | Jyonouchi H, et al. (1994) Nucleotide-free diet impairs T-helper cell functions in antibody production in response to T-dependent antigens in normal C57B1/6 mice. J Nutr 124(4):475-84 |
| abstractText | We have previously shown that polynucleotides enhance in vitro antibody production in response to T-dependent antigens. This study examines the importance of dietary nucleotides on in vivo antibody production in response to antigen stimuli. C57Bl/6 mice fed a nucleotide-free diet for more than 3 wk demonstrated significantly lower antibody production to T-dependent antigens both in vivo and in vitro compared with control mice fed a nonpurified diet. Numbers of immunoglobulin M and G secreting cells in the spleen were also lower in mice fed a nucleotide-free diet after the challenge of T-dependent antigens compared to the controls. Responses to T-independent antigens remained intact in mice fed a nucleotide-free diet. T-helper cells from mice fed a nucleotide-free diet were less capable of inducing T-dependent antibody production in vitro compared with those from mice fed a nonpurified diet. Intraperitoneal injection of a mononucleotide-nucleoside mixture restored T-helper cell functions and T-dependent antibody production in mice fed a nucleotide-free diet. These results may indicate the importance of dietary nucleotides in maintaining optimal T-helper cell functions for humoral immune responses in response to T-dependent antigens. |
