Primary Identifier | MGI:6377429 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Gm28729 |
Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
Is Recombinase | false | Is Wild Type | false |
Project Collection | IMPC |
molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences GTTTCTAGTGCTGCACCACT and GGCTCAACTTGGGAACACAT, which resulted in a 940 bp deletion beginning at Chromosome 9 position 96,517,356 bp and ending after 96,518,295 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001318241 (exon 4) and 791 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 111 and early truncation 3 amino acids later. |