| First Author | Derynck R | Year | 1984 |
| Journal | Cell | Volume | 38 |
| Issue | 1 | Pages | 287-97 |
| PubMed ID | 6088071 | Mgi Jnum | J:15799 |
| Mgi Id | MGI:63910 | Doi | 10.1016/0092-8674(84)90550-6 |
| Citation | Derynck R, et al. (1984) Human transforming growth factor-alpha: precursor structure and expression in E. coli. Cell 38(1):287-97 |
| abstractText | Transforming growth factor-alpha (TGF-alpha) is secreted by many human tumors and can induce the reversible transformation of nontransformed cell lines. Using long synthetic deoxyoligonucleotides as hybridization probes we isolated an exon coding for a portion of TGF-alpha from a human genomic DNA library. Utilizing this exon as a probe, a cell line derived from a human renal cell carcinoma was identified as a source of TGF-alpha mRNA. A cloned TGF-alpha cDNA was isolated from a cDNA library prepared using RNA from this cell line, and was found to encode a precursor polypeptide of 160 amino acids. The 50 amino acid mature TGF-alpha produced by expression of the appropriate coding sequence in E. coli binds to the epidermal growth factor receptor and induces the anchorage independence of normal mammalian cells in culture. |
