Primary Identifier | MGI:3054940 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Prkci |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 9 and intron 9, containing the ATP binding site and the splice acceptor site for exon 10, were replaced with a PGK-neobpA cassette in the opposite transcriptional orientation. RT-PCR from day 7.5 mutant embryos showed there was no transcript. |