Primary Identifier | MGI:5500170 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Myod1 |
Transmission | Germline | Strain of Origin | 129/Sv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A 1.9-kb genomic fragment containing the 714-bp downstream regulatory region was replaced with a PGKneo-positive selection cassette flanked by loxP sites. The neo selection cassette was subsequently removed by transient cre expression. Nothern blot analysis confirmed restored transcript expression levels. |