First Author | von Deimling OH | Year | 1991 |
Journal | Electrophoresis | Volume | 12 |
Issue | 6 | Pages | 446-7 |
PubMed ID | 1889396 | Mgi Jnum | J:27015 |
Mgi Id | MGI:74433 | Doi | 10.1002/elps.1150120614 |
Citation | von Deimling OH, et al. (1991) Demonstration of murine pancreas elastase and its interstrain variation by isoelectric focusing. Electrophoresis 12(6):446-7 |
abstractText | Isoelectric focusing between pH 9 and 11 was used for separation of murine pancreas proteases. One of these proteases is characterized by its preference for N-acetyl-L-alanine-alpha-naphthylester as substrate and by its genetic linkage to bt, a coat color marker of chromosome 15. This protease was identified as elastase, and is probably elastase-1 (ELA-1). Because of the simple procedure and the excellent reproducibility of the focusing pattern, ELA-1 is recommended as a useful marker for mouse chromosome 15. |