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Publication : Demonstration of murine pancreas elastase and its interstrain variation by isoelectric focusing.

First Author  von Deimling OH Year  1991
Journal  Electrophoresis Volume  12
Issue  6 Pages  446-7
PubMed ID  1889396 Mgi Jnum  J:27015
Mgi Id  MGI:74433 Doi  10.1002/elps.1150120614
Citation  von Deimling OH, et al. (1991) Demonstration of murine pancreas elastase and its interstrain variation by isoelectric focusing. Electrophoresis 12(6):446-7
abstractText  Isoelectric focusing between pH 9 and 11 was used for separation of murine pancreas proteases. One of these proteases is characterized by its preference for N-acetyl-L-alanine-alpha-naphthylester as substrate and by its genetic linkage to bt, a coat color marker of chromosome 15. This protease was identified as elastase, and is probably elastase-1 (ELA-1). Because of the simple procedure and the excellent reproducibility of the focusing pattern, ELA-1 is recommended as a useful marker for mouse chromosome 15.
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