| Primary Identifier | MGI:3641577 | Allele Type | Transgenic |
| Attribute String | Recombinase | Gene | Tg(Pgk2-cre)24Shb |
| Strain of Origin | C57BL/6 | Is Recombinase | true |
| Is Wild Type | false |
| description | Two transgenic mice derived from injected oocytes transmitted the transgene to their progeny. The resultant transgenic mouse lines were designated 3 and 24. Because Cre-mediated recombination sometimes failed to occur in double transgenic progeny of line 3, only line 24 was used. |
| molecularNote | Nucleotides -1404 through +61 of the spermatocyte-specific Pgk2 promoter region were joined to the cDNA encoding Cre recombinase followed by a rabbit globin polyadenylation signal. Expression of cre mRNA in testes was demonstrated by RT-PCR analysis. Double transgenic progeny of a cross of mice with this transgene to animals carrying a loxP-flanked EGFP reporter gene exhibit EGFP fluorescence in spermatocytes and spermatids, but not in spermatogonia or somatic cells of the testis. |
