| First Author | Bundschu K | Year | 2006 |
| Journal | Gene Expr Patterns | Volume | 6 |
| Issue | 3 | Pages | 247-55 |
| PubMed ID | 16378760 | Mgi Jnum | J:136790 |
| Mgi Id | MGI:3796973 | Doi | 10.1016/j.modgep.2005.08.003 |
| Citation | Bundschu K, et al. (2006) Tissue-specific Spred-2 promoter activity characterized by a gene trap approach. Gene Expr Patterns 6(3):247-55 |
| abstractText | Spreds (Sprouty-related proteins with an Ena/Vasodilator-stimulated phosphoprotein homology-1 domain) are a new protein family inhibiting the mitogen-activated protein kinase (MAPK) signaling pathway. Different RNA and protein studies already revealed an almost ubiquitous Spred-2 expression pattern. But until now, only few data were available on the in situ Spred-2 promoter activity. Here, we show a detailed in situ analysis of a mouse strain with a trapped Spred-2 gene, bringing a beta-galactosidase and neomycin fusion gene (beta-geo) under the control of the endogenous Spred-2 promoter. This allowed us to monitor Spred-2 promoter activity in practically every organ and their corresponding sub-compartments. X-Gal staining of newborn and adult mice revealed a nearly congruent Spred-2 promoter activity pattern. Our detailed data provide information for further studies of the still enigmatic physiological functions of Spred-2 in various organs by identifying the tissues with strong Spred-2 promoter activity. |
